Continuous Monoxenic Culture of Tetrahymena pyriformis
نویسندگان
چکیده
منابع مشابه
Two-dimensional gravitactic bioconvection in a protozoan (Tetrahymena pyriformis) culture.
Gravitactic bioconvective patterns created by Tetrahymena pyriformis in a Hele-Shaw apparatus were realized and compared with theoretical results. There were found to be two thresholds for bio-convection development: the first indicates the transition from the diffusion to the steady convection state; the second corresponds to the transition from the steady to the unsteady convection state. The...
متن کاملRibosome Synthesis in Tetrahymena Pyriformis
The cellular site of synthesis of ribosomal RNA in Tetrahymena pyriformis was studied by analyzing the purified nuclear and cytoplasmic RNA from cells pulse labeled with uridine-(3)H. The results of studies using zonal centrifugation in sucrose density gradients show that the ribosomal RNA is synthesized in the nucleus as a large precursor molecule sedimenting at 35S. The 35S molecule undergoes...
متن کاملStudies of Membrane Formation in Tetrahymena Pyriformis
A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals ...
متن کاملReactivation of Glycerinated Cilia from Tetrahymena Pyriformis
The ease with which Tetrahymena can be grown in relatively large quantity makes it an exceptionally useful source of cilia for biochemical study. Several methods of isolating the cilia have been described (3, 11). Although excellent for many purposes, these procedures all suffer from the limitation that the isolated cilia seem incapable of being reactivated by ATP. This paper describes a method...
متن کاملPurine-metabolizing enzymes of Tetrahymena pyriformis.
Except for the recent report of Friedkin on pyrimidine nucleoside phosphorylase (2) and several papers from this laboratory dealing with ribonuclease and deoxyribonuclease (3, 4), little information is available concerning the occurrence of nucleolytic and nucleosynthetic enzymes in the ciliated protozoan, Tetrahymena pyrif0rmis.l This is in contrast to a number of studies carried out on the pu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1971
ISSN: 0022-1287
DOI: 10.1099/00221287-66-1-95